Glucose Uptake at Rest and During Contraction in Isolated Dog Skeletal Muscle1

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Abstract
Costin, J. C., B. Saltin, N. S. Skinner, Jr. and G. Vastagh,Glucose uptake at rest and during contraction in isolated dog skeletal muscle. Acta physiol. scand. 1971.81. 124–137.In an in situ isolated canine gracilis muscle preparation the a‐v difference for glucose, oxygen, and lactate has been studied at rest and during stimulation. Both free flow (systemic blood flow) and constant flow perfusion (blood from reservoir) have been applied and in both sets of experiments the blood flow has been determined. In the constant flow experiments no significant difference in the glucose uptake could be detected between stimulation as compared with rest. In the free flow experiments the same stimulation frequency produced a significant uptake of glucose (≅ 20 μgglucose per g muscle and minute), which could account for approximately 10 per cent of the total energy output and 20–30 per cent of the total carbohydrate utilization. It is suggested that the difference between the free flow and the constant flow experiments was due to the fact that, in the free flow experiments where the muscle is perfused with the systemic blood flow, some humoral factor is present which is necessary for the glucose to penetrate the cell barrier. It was also possible to demonstrate a transient glucose uptake in the muscle in the constant flow experiments,i.e. when the muscle was suddenly perfused with blood containing a high glucose concentration (≅ 150 mg/100 ml blood).