Growth characteristics of feline panleukopenia virus in synchronized kitten kidney cells.

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Abstract
The replication of feline panleukopenia virus (FPLV) in synchronized kitten kidney (KK) cells was studied. The adsorption rates of [3H]-thymidine-labeled FPLV to each of the G1, early S and M phases of cell cycle were almost the same. The KK cells maintained in the G1 phase did not support the replication of FPLV during the 12-36 h of the observation period. When the cell cycle was allowed to proceed by trypsinizing G1 phase cells at 0, 6 and 12 h after virus infection, a slight replication of the virus was observed. A high replication of FPLV was obtained from the cells infected in the early S phase. Early S phase cells arrested with 2 mM thymidine (TdR) supported the growth of FPLV when the initiation of the cell cycle was stimulated by the removal of TdR within 12 h after infection. No replication of FPLV was observed in the M phase cells. FPLV replication requires the DNA synthesizing system of KK cells.