Studies on viomycin. XV. Comparative study on the specificities of two anti-viomycin antisera by enzyme immunoassay.

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Abstract
A new viomycin-bovine serum albumin conjugate was characterized and 19 viomycin molecules are coupled with 1 molecule of the carrier protein. Enyzme labeling of viomycin with .beta.-D-galactosidase was performed by a continuous 2-step process using a maleimide succinimidyl ester type cross-linker. The optical conditions for enzyme immunoassay of viomycin using the new anti-viomycin antiserum and the enzyme labeled (double antibody method) were studied and a procedure with satisfactory accuracy and precision was developed. The specificities of 2 anti-viomycin antisera was compared by the enzyme immunoassay technique. Evidence that the enzyme immunoassay procedure is preferable to radioimmunoassay for a comparative study is also presented.