Isolation of Monoaminergic Synaptosomes from Rat Brain by Immunomagnetophoresis
- 1 May 1991
- journal article
- Published by Wiley in Journal of Neurochemistry
- Vol. 56 (5) , 1569-1580
- https://doi.org/10.1111/j.1471-4159.1991.tb02053.x
Abstract
Monoaminergic synaptosomes have been isolated and purified from rat brain by immunomagnetophoresis. This novel technique uses magnetic beads to which Protein A is bound. Noradrenergic, dopaminergic, and serotonergic synaptosomes (previously cell-surface labelled with anti-dopamine-beta-hydroxylase, anti-tyrosine hydroxylase, and anti-tryptophan hydroxylase, respectively) may be isolated in a highly purified state. The synaptosomal subpopulations are recovered in a viable metabolic state and show glucose-stimulated respiration and Ca2(+)-dependent neurotransmitter release. A novel subtype of dopamine-beta-hydroxylase was found in dopaminergic terminals. No evidence for glutamate corelease from monoaminergic synaptosomes was obtained.Keywords
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