A rapid method, using octadecasilyl-silica, for the extraction of certain peptides from tissues
- 1 December 1978
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 175 (3) , 1139-1141
- https://doi.org/10.1042/bj1751139
Abstract
Peptides can be recovered from [rat] tissues [kidney, liver, small intestine, muscle] by homogenization in a carefully selected extraction medium followed by adsorption from supernatant on a small bed of octadecasilyl-silica. Recoveries of corticotropins and somatostatin added to a variety of tissues were quantitative, and the peptides undamaged as determined by high-pressure liquid chromatography.This publication has 5 references indexed in Scilit:
- EARLY FATE OF SOMATOSTATIN IN THE CIRCULATION OF THE RAT AFTER INTRAVENOUS INJECTIONJournal of Endocrinology, 1978
- Use of octadecasilyl-silica for the extraction and purification of peptides in biological samples. Application to the identification of circulating metabolites of corticotropin-(1-24)-tetracosapeptide and somatostatin in vivoBiochemical Journal, 1977
- The synthesis of tritium-labelled human corticotropin of high specific radioactivityBiochemical Journal, 1977
- Fluorometric assay of vasopressin and oxytocin: a general approach to the assay of peptides in tissues.Proceedings of the National Academy of Sciences, 1976
- Tritiated peptides. Part 3. Synthesis of [4-3H-Phe7]-β-corticotrophin-(1–24)-tetracosapeptideJournal of the Chemical Society, Perkin Transactions 1, 1976