Monoclonal anti-mouse macrophage antibodies recognize the globular portions of C1q, a subcomponent of the first component of complement.
Open Access
- 1 July 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Immunology
- Vol. 133 (1) , 400-404
- https://doi.org/10.4049/jimmunol.133.1.400
Abstract
One of seven monoclonal antibodies generated against mouse macrophages (M phi) was found to recognize isolated heterologous C1q. This antibody was shown to be cytotoxic and to react in a strain-independent way with mouse M phi derived from bone marrow cells as well as with M phi from the peritoneal cavity; it did not react, however, with mouse granulocytes, thymocytes, or T and B lymphocytes. The hemolytic activity of fluid phase C1q was inhibited to 50% at a 2 X 10(-4) dilution of hybridoma supernatant, whereas a 100-fold higher concentration was required to inhibit C1q bound to immune complexes ( EAC1q ) to the same extent. It was demonstrated that this antibody recognizes the isolated globular, Fc-binding portions of the C1q molecule and reacts with the A and B chains. Because M phi have been shown to synthesize C1q, the Fc-recognizing subcomponent of the first component of complement, evidence was provided that endogeneous C1q can serve as an Fc receptor on M phi during secretion. This fact was demonstrated by a dose-dependent inhibition of Fc-receptor activity for EIgG by the F(ab')2 fragment of this monoclonal antibody. These experiments further support the concept that C1q produced by M phi functions on the surface as an Fc-recognizing molecule before it is released and incorporated into the macromolecular complex of serum C1.This publication has 11 references indexed in Scilit:
- The relationship between collagen and C1q biosynthesis in cultured human fibroblastsBiochemical Journal, 1982
- Conformational changes in C1q after binding to immune complexes: detection of neoantigens with monoclonal antibodies.The Journal of Immunology, 1982
- Immunofluorescence studies on the subcomponents of the first component of complement (C1): Detection of C1q and C1s in different cells of biopsy material and on Human as well as on guinea pig peritoneal macrophagesImmunobiology, 1981
- Induction of anamnestic T cell proliferation by antigen‐pulsed, bone marrow‐derived macrophagesEuropean Journal of Immunology, 1980
- Purificaton of a functional mouse Fc receptor through the use of a monoclonal antibody.The Journal of Experimental Medicine, 1980
- Differentiation of hemolytically active fluid-phase and cell-bound human C1q by an ant venom-derived polysaccharide.The Journal of Immunology, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- Detection of human histocompatibility (HLA) antigens with an indirect rosette microassayJournal of Immunological Methods, 1979
- Comparison of the direct antiglobulin rosetting reaction with the mixed antiglobulin rosetting reaction for the detection of immunoglobulin on lymphocytesJournal of Immunological Methods, 1977
- CHROMATOGRAPHIC RESOLUTION OF THE FIRST COMPONENT OF HUMAN COMPLEMENT INTO THREE ACTIVITIESThe Journal of Experimental Medicine, 1963