Polyethylene Glycols as Embedding Media in Histochemical Work
- 1 November 1952
- journal article
- research article
- Published by BMJ in Journal of Clinical Pathology
- Vol. 5 (4) , 345-349
- https://doi.org/10.1136/jcp.5.4.345
Abstract
Appropriately fixed tissues 2-3 mm. thick are blotted and immersed in 2 changes of "carbo-wax 1000" at 40[degree]C for 1 hr. each and then embedded in freshly melted "carbowax 1000". The blocks are enclosed in a petri dish and cooled for 15 mins. in an ice box. Mounted sections are dried overnight at 37[degree]C. Glycogen, fat, nucleoproteins, acid and alkaline phosphatase, oxidase and peroxidase are demonstrable histochemically in the sections. Blocks are preserved after wrapping in cellophane paper in an air-tight glass jar, preferably in an ice box.Keywords
This publication has 11 references indexed in Scilit:
- Micromethods and Their Application in the Study of Enzyme Distribution in Tissues and CellsPhysiological Reviews, 1951
- Histological demonstration of lipids in tissue after dehydration and embedding in a polyethylene glycol.1951
- Propylene and Ethylene Glycol as Solvents for Sudan IV and Sudan Black BStain Technology, 1951
- A general method for preparing histologic sections with a water-soluble wax.1950
- Carbowax Embedding for Obtaining Thin Tissue Sections and Study of Intracellular LipidsStain Technology, 1950
- A Rapid Imbedding Technic for Histologic Sections Employing a Water Soluble Wax11This study was supported by a grant-in-aid from the United States Public Health Service.Journal of Investigative Dermatology, 1949
- HISTOCHEMICAL METHODS APPLIED TO HEMATOLOGYBlood, 1948
- Effect of Acetone and Alcohol Fixation and Paraffin Embedding on Activity of Acid and Alkaline Phosphatases in Rat TissuesScience, 1948
- Acid Phosphatase and Lipids in the Mast Cells of the RatScience, 1947
- Comparison of Methods for Demonstrating Glycogen MicroscopicallyStain Technology, 1939