Carcinogen-mediated induction of SV40 DNA synthesis in SV40 transformed Chinese hamster embryo cells
- 1 January 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Carcinogenesis: Integrative Cancer Research
- Vol. 2 (5) , 417-423
- https://doi.org/10.1093/carcin/2.5.417
Abstract
Exposure of SV40-transformed Chinese hamster embryo cells (line CO50) to a series of physical and chemical carcinogens (including activation-dependent and activation-independent varieties) resulted in the induction of viral DNA synthesis. The carcinogen mediated amplification of SV40 DNA was demonstrated by a highly sensitive in situ hybridization procedure for the detection of cells synthesizing SV40 DNA. Treatment of CO50 cells with an inhibitor of polycyclic hydrocarbon metabolism (7,8-benzoflavone) prior to the application of benzo[a]pyrene or 7,12-dimethyl-benz[a]anthracene prevented the induction of SV40 DNA synthesis, indicating that the induction depends upon the metabolic activation of these compounds. Non-carcinogenic hydrocarbons were inactive under this assay. Two different protocols for determining the inducing potential of a compound are presented. The properties of this test and its possible use as a short-term assay for potential carcinogens is discussed. The possibility that the induction of SV40 DNA synthesis is a reflection of a general gene amplification phenomenon mediated by carcinogens is discussed.Keywords
This publication has 8 references indexed in Scilit:
- Indiscriminate recombination in simian virus 40-infected monkey cells.Proceedings of the National Academy of Sciences, 1980
- Amplified dihydrofolate reductase genes in unstably methotrexate-resistant cells are associated with double minute chromosomes.Proceedings of the National Academy of Sciences, 1979
- Short-term tests for carcinogens and mutagensMutation Research/Reviews in Genetic Toxicology, 1979
- Sequences of five potential recombination sites encoded close to an immunoglobulin kappa constant region gene.Proceedings of the National Academy of Sciences, 1979
- Sequences at the somatic recombination sites of immunoglobulin light-chain genesNature, 1979
- Evidence for the progressive nature of neoplastic transformation in vitro.Proceedings of the National Academy of Sciences, 1978
- Relationship between somatic mutation and neoplastic transformationProceedings of the National Academy of Sciences, 1978
- Identification of mutagenic metabolites of benzo(a)pyrene in mammalian cells.Proceedings of the National Academy of Sciences, 1976