Sequences at the somatic recombination sites of immunoglobulin light-chain genes
- 1 July 1979
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 280 (5720) , 288-294
- https://doi.org/10.1038/280288a0
Abstract
The entire nucleotide sequence of a 1.7-kilobase [mouse] embryonic DNA fragment containing 5 joining (J) DNA segments for mouse immunoglobulin .KAPPA. chain gene was determined. Each J DNA segment can encode amino acid residues 96-108. Comparison of 1 of the 5 J DNA sequences with those of an embryonic variable (V) gene and a complete .KAPPA. chain gene permitted localization of a precise recombination site. The 5''-flanking regions of J DNA segments could form an inverted stem structure with the 3''-non-coding region of embryonic V genes. This hypothetical structure and gel-blotting analysis of total embryo and myeloma DNA suggest that the somatic recombination may be accompanied by excision of an entire DNA segment between a V gene and a J DNA segment. Antibody diversity may in part be generated by modulation of the precise recombination sites.Keywords
This publication has 31 references indexed in Scilit:
- DNA sequence analysis of Tn10 insertions: Origin and role of 9 bp flanking repetitions during Tn10 translocationCell, 1979
- Domains and the hinge region of an immunoglobulin heavy chain are encoded in separate DNA segmentsNature, 1979
- Multiplicity of germline genes specifying a group of related mouse κ chains with implications for the generation of immunoglobulin diversityNature, 1978
- The transposon Tn9 generates a 9 bp repeated sequence during integrationCell, 1978
- Sequences of mouse immunoglobulin light chain genes before and after somatic changesCell, 1978
- The relationship between function and DNA sequence in an intercistronic regulatory region in phage λNature, 1978
- DNA sequence at the integration sites of the insertion element IS1Cell, 1978
- Two adenovirus mRNAs have a common 5′ terminal leader sequence encoded at least 10 kb upstream from their main coding regionsCell, 1977
- Improved derivative of a phage λ EK2 vector for cloning recombinant DNANature, 1976
- Proteolytic Cleavage of Native DNA Polymerase into Two Different Catalytic FragmentsEuropean Journal of Biochemistry, 1971