Ultrastructural localization of HMB‐45 binding sites
- 1 December 1991
- journal article
- Published by Wiley in Journal of Cutaneous Pathology
- Vol. 18 (6) , 432-435
- https://doi.org/10.1111/j.1600-0560.1991.tb01380.x
Abstract
Three malignant melanomas, two melanoma metastases, two junctional dysplastic nevi, and normal skin were embedded in Lowicryl. Ultrathin sections were incubated with HMB-45 and a gold-labeled anti-mouse antibody. Gold particles indicating the presence of HMB-45 were found in melanosomes Stage 1 and 2 and in the non-melanized portion of melanosomes Stage 3. Melanosomes Stage 4 and melanosome complexes in keratinocytes, as well as in melanophages, were consistently negative. No specific labelling with HMB-45 was seen in eccrine glands of normal skin.Keywords
This publication has 9 references indexed in Scilit:
- Antibody HMB-45 Identifies the Cells of Blue NeviThe American Journal of Surgical Pathology, 1990
- Ultrastructural Localization of Lectin-Binding Sites in Normal SkinJournal of Investigative Dermatology, 1990
- The appearance, density and distribution of melanocytes in human embryonic and fetal skin revealed by the anti-melanoma monoclonal antibody, HMB-45Brain Structure and Function, 1989
- Breast Carcinoma with Positive Results for Melanoma Marker (HMB-45): HMB-45 Immunoreactivity in Normal and Neoplastic BreastAmerican Journal of Clinical Pathology, 1989
- HMB-45 Staining of Dysplastic NeviThe American Journal of Surgical Pathology, 1989
- Recognition of malignant melanoma by monoclonal antibody HMB‐45. An immunohistochemical study of 200 paraffin‐embedded cutaneous tumorsJournal of Cutaneous Pathology, 1988
- Distribution of melanoma specific antibody (HMB-45) in benign and malignant melanocytic tumoursVirchows Archiv, 1988