Comparison of liquid growth media for Legionella pneumophila

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Abstract
Ten liquid media were compared under standard conditions for their ability to support the growth of L. pneumophila. Modified gonococcal-ferric cysteine broth (without NaCl) supplemented with 1% yeast extract yielded the best overall growth of the 1 strain of L. pneumophila examined. Growth rates were independent of pH changes which occurred during incubation. The growth rates of 10 different strains of L. pneumophila were compared in this medium. There appeared to be little difference in the growth rates of strains passaged frequently or infrequently, or between environmental and clinical (human) isolates. Moderate aeration resulted in a faster growth rate and in .apprx. 1 log10 higher final cell concentration as compared to a static broth culture. Thus, there are moderate to marked differences among the various media described in the literature. No liquid medium yet developed supports rapid growth of L. pneumophila incubated without shaking.