The Most Abundant Protease Inhibitor in Potato Tuber (Cv. Elkana) Is a Serine Protease Inhibitor from the Kunitz Family
- 12 July 2003
- journal article
- Published by American Chemical Society (ACS) in Journal of Agricultural and Food Chemistry
- Vol. 51 (17) , 5001-5005
- https://doi.org/10.1021/jf0212486
Abstract
The gene of the most abundant protease inhibitor in potato cv. Elkana was isolated and sequenced. The deduced amino acid sequence of this gene showed 98% identity with potato serine protease inhibitor (PSPI), a member of the Kunitz family. Therefore, the most abundant protease inhibitor was considered to be one of the isoforms of PSPI. The PSPI group represents approximately 22% of the total amount of proteins in potato cv. Elkana and is composed of seven different isoforms that slightly differ in isoelectric point. Antibodies were raised against the two most abundant isoforms of PSPI. The binding of these antibodies to PSPI isoforms and protease inhibitors from different groups of protease inhibitor in potato showed that approximately 70% of the protease inhibitors present in potato juice belong to the Kunitz family.Keywords
This publication has 16 references indexed in Scilit:
- Relative Abundance and Inhibitory Distribution of Protease Inhibitors in Potato Juice from cv. ElkanaJournal of Agricultural and Food Chemistry, 2001
- Gapped BLAST and PSI-BLAST: a new generation of protein database search programsNucleic Acids Research, 1997
- The octadecanoid signalling pathway in plants mediates a response to ultraviolet radiationNature, 1996
- Starch synthesis-, and tuber storage protein genes are differently expressed in Solanum tuberosum and in Solanum brevidensFEBS Letters, 1996
- Posttranslational Modification of an Isoinhibitor from the Potato Proteinase Inhibitor II Gene Family in Transgenic Tobacco Yields a Peptide with Homology to Potato Chymotrypsin Inhibitor IPlant Physiology, 1994
- Isolation and sequence analysis of the genomic DNA fragment encoding an aspartic proteinase inhibitor homologue from potato (Solanum tuberosum L.)Plant Molecular Biology, 1992
- Characterization of Aspartic Proteinase Inhibitors from Potato at the Gene, cDNA and Protein LevelsBiological Chemistry Hoppe-Seyler, 1992
- The amino acid sequence of a novel inhibitor of cathepsin D from potatoFEBS Letters, 1990
- Primary structure of cathepsin D inhibitor from potatoes and its structure relationship to soybean trypsin inhibitor familyFEBS Letters, 1989
- Molecular characterization of a wound-inducible inhibitor I gene from potato and the processing of its mRNA and proteinPlant Molecular Biology, 1987