Analysis of mutations introduced into the chromosomal immunoglobulin ? gene

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Abstract
We have introduced a pSV2neo-derived vector that contains a 2-base-pair (bp) deletion in its immunoglobulin μ gene constant region into hybridoma cells bearing a single copy of the wild-type chromosomal immunoglobulin μ gene. Homologous recombination between the transferred mutant Cμ region and the wild-type chromosomal Cμ region is expected to introduce the 2-bp deletion into the chromosomal μ gene, generating recombinant cells synthesizing noncytolytic IgM. Analysis of the DNA in independent noncytolytic transformants indicates that in one case the μ gene has the structure expected for correct homologous recombination. Unexpectedly, the remaining transformants, bear chromosomal μ gene deletions.