THE ISOLATION OF TWO CORTICOSTEROIDS FROM CATTLE BILE1

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Abstract
THE presence of metabolites of adrenal cortical steroids in bile has been demonstrated in studies utilizing steroids labeled with tritium (1) or with C14 (2-10). In the rodent, excretion of isotopic metabolites by the biliary route was predominant (1-5), while in man, only a small percentage of the administered radioactivity was excreted by this pathway (6–8) or detected in the feces (7–10). In the present paper, the isolation and identification of tetrahydrocortisol (THF) and tetrahydrocortisone (THE) from cattle bile are described. These steroids, probably metabolites of adrenal cortical steroids in this species, were obtained from bile that had been treated with a culture fluid containing bacterial β-glucuronidase.