Construction and use ofAgrobacterium tumefaciens binary vectors withA. tumefaciens C58 T-DNA genes
- 1 June 1992
- journal article
- Published by Springer Nature in Folia Microbiologica
- Vol. 37 (3) , 227-230
- https://doi.org/10.1007/bf02933153
Abstract
Five plant morphoregulatory genes were isolated from theAgrobacterium tumefaciens Ti plasmid and binary plasmid vectors for plant transformation with these genes were constructed. All vectors have a similar structure with T-DNA borders, RK2 origin of replication and chimeric kanamycin resistance gene for the selection of transformed plant tissues. Over twenty vectors with single and combined morphoregulatory genes were constructed and their effects after tobacco tissue transformation studied.Keywords
This publication has 25 references indexed in Scilit:
- Transgenic Tobacco plants with T-DNA Phytohormone synthesis genesBiologia plantarum, 1991
- Potato transformation by T-DNA Cytokinin synthesis geneBiologia plantarum, 1990
- A Simple and General Method for Transferring Genes into PlantsScience, 1985
- A new technique for genetic engineering of Agrobacterium Ti plasmidPlasmid, 1983
- A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmidNature, 1983
- Genetic analysis of T-DNA transcripts in nopaline crown gallsCell, 1983
- Size, location and polarity of T-DNA-encoded transcripts in nopaline crown gall tumors; Common transcripts in octopine and nopaline tumorsCell, 1983
- Molecular cloning of overlapping segments of the nopaline Ti-plasmid pTiC58 as a means to restriction endonuclease mappingPlasmid, 1980
- Conditions for mutagenesis by N-methyl-N′-nitro-N-nitrosoguanidine and relationships of A. tumefaciens mutants to crown-gall tumor inductionMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1972
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962