The isolation of d-glycero-d-galactoheptose and other sugar components from the specific polysaccharide of Chromobacterium violaceum (BN)

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Abstract
A preparation of the somatic antigen of C. violaceum (BN) was obtained by extraction of acetone-dried cells with trichloro-acetic acid. By hydrolysis with 1% (v/v) acetic acid at 100 C, the somatic antigen was separated into degraded polysaccharide and lipoprotein components. The degraded polysaccharide was hydrolyzed to release its component sugars; the hydrolysate was passed through an ion-exchange resin. D-Glucosamine hydrochloride was isolated from an acidic eluate of the resin column. The neutral sugars, obtained by eluting the resin column with water before the acid elution, were separated on a column of powdered cellulose with 95% (v/v) aqueous acetone as the developing solvent. From the earlier fractions of the cellulose-column effluent L-rhamnose was isolated. From the later fractions of the cellulose-column effluent, D-glycero-D-galacto-heptose was isolated; this sugar was not previously known to occur in nature.