Horizontal polyacrylamide gel electrophoresis for the separation of DNA fragments
- 1 January 1994
- journal article
- research article
- Published by Wiley in Electrophoresis
- Vol. 15 (1) , 153-158
- https://doi.org/10.1002/elps.1150150126
Abstract
A discontinuous borate/formate buffer system is presented for horizontal polyacrylamide gel electrophoresis of DNA fragments. The resolution potential of the system could be altered by changing the total monomer concentration (5–9%T), the concentration of the crosslinker piperazine diacrylamide (2–5%CPDA), as well as the concentration of formate in the gel (40–120 mM), the leading ion of the buffer system. The separation of DNA fragments would be improved by increasing the migration distance from 22 to 28 cm. This discontinuous polyacrylamide gel electrophoresis system proved highly reproducible.Keywords
This publication has 20 references indexed in Scilit:
- Long-term T-cell reconstitution after hematopoietic stem-cell transplantation in primary T-cell–immunodeficient patients is associated with myeloid chimerism and possibly the primary disease phenotypeBlood, 2007
- Improved separation of PCR amplified VNTR alleles by a vertical polyacrylamide gel electrophoresisInternational journal of legal medicine, 1993
- Population genetics and forensic efficiency data of 4 AMPFLP'sInternational journal of legal medicine, 1992
- Discontinuous buffer system for polyacrylamide and agarose gel electrophoresis of DNA fragmentsElectrophoresis, 1991
- Japanese Pharmaceutical Biotechnology: Perception vs. RealityNature Biotechnology, 1989
- Enzymatic Amplification of β-Globin Genomic Sequences and Restriction Site Analysis for Diagnosis of Sickle Cell AnemiaScience, 1985
- Selected buffer systems for moving boundary electrophoresis on gels at various pH values, presented in a simplified mannerElectrophoresis, 1983
- Ultrasensitive Stain for Proteins in Polyacrylamide Gels Shows Regional Variation in Cerebrospinal Fluid ProteinsScience, 1981
- Some aspects of the use of “continuous” and “discontinuous” buffer systems in polyacrylamide gel electrophoresisAnalytical Biochemistry, 1965