Heat shock proteins of higher plants

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Abstract
The pattern of protein synthesis changes rapidly and dramatically when the growth temperature of soybean [Glycine max cv. Wayne] seedling tissue is increased from 28.degree. C (normal) to .apprx. 40.degree. C (heat shock). The synthesis of normal proteins is greatly decreased and a new set of proteins, heat shock proteins, is induced. The heat shock proteins of soybean consist of 10 new bands on 1-dimensional [sodium dodecylsulfate] gels; a more complex pattern is observed on 2-dimensional gels. When the tissue is returned to 28.degree. C after 4 h at 40.degree. C, there is progressive decline in the synthesis of heat shock proteins and reappearance of a normal pattern of synthesis by 3 or 4 h. In vitro translation of poly(A)+RNA isolated from tissues grown at 28.degree. and 40.degree. C shows that the heat shock proteins are translated from a new set of mRNA induced at 40.degree. C; the abundant class mRNA for many of the normal proteins persist even though they are translated weakly in vivo at 40.degree. or 42.5.degree. C. The heat shock response in soybean appears similar to the much-studied heat shock phenomenon in Drosophila.