Sodium pyrophosphate inhibition of RNA.DNA hybrid degradation by reverse transcriptase.
- 1 November 1978
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 75 (11) , 5329-5333
- https://doi.org/10.1073/pnas.75.11.5329
Abstract
Sodium pyrophosphate inhibits synthesis of anticomplementary DNA during a reverse transcriptase (RNA-directed DNA nucleotidyltransferase, EC 2.7.7.7) [from avian myeloblastosis virus] catalyzed reaction. In the presence of pyrophosphate, the complementary DNA remains stably complexed to the RNA template. In the absence of pyrophosphate, the DNA .cntdot. RNA hybrid template is degraded and anticomplementary DNA is synthesized. High concentrations of additives containing phosphodiester bonds appear to inhibit the RNase H activity (hybrid nuclease, EC 3.1.4.34) of the reverse transcriptase, thereby preventing formation of RNA primers necessary for the synthesis of anticomplementary DNA. [Poliovirus RNA and phage f1 DNA were used].This publication has 16 references indexed in Scilit:
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