HUMAN PERIPHERAL LYMPHOCYTES BEARING SURFACE-IMMUNOGLOBULIN DO NOT HAVE READILY DETECTABLE FC-RECEPTORS

  • 1 January 1978
    • journal article
    • research article
    • Vol. 33  (2) , 332-339
Abstract
The question of whether human peripheral B [bone marrow-derived] lymphocytes have Fc receptors was examined directly by double-label immunofluorescent techniques utilizing assays for detection of Fc receptors, surface immunoglobulin and complement receptors. Fc receptors were detected by indirect immunofluorescence after incubation with soluble antigen-antibody complexes. Complement receptors were detected by the binding of fluoresceinated bacteria coated with complement. Most surface immunoglobulin-bearing, complement-receptor positive lymphocytes did not bind soluble antigen-antibody complexes. Most cells which readily bound soluble complexes did not have surface immunoglobulin or complement receptors. Most peripheral B lymphocytes do not have easily detectable Fc receptors and most Fc receptor-bearing lymphocytes do not have B cell markers.