UV‐B‐ and oxidative stress‐induced increase in nicotinamide and trigonelline and inhibition of defensive metabolism induction by poly(ADP‐ribose)polymerase inhibitor in plant tissue

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Abstract
Nicotinamide and trigonelline contents increased in Catharanthus roseus tissue culture after exposure to 2,2′azobis(2‐amidinopropane) dihydrochloride (AAPH) or vanadylsulfate and in Pisum sativum leaves after exposure to UV‐B radiation. Vanadylsulfate increased phenylalanine ammonia‐lyase (PAL) activity and the content of reduced and oxidized glutathione in C. roseus tissue culture. The increases in PAL activity caused by 2 mM AAPH or 0.2 mM vanadylsulfate were prevented by 0.1 mM 3‐aminobenzamide (3‐AB), an inhibitor of poly(ADP‐ribose)polymerase. Present results support the hypothesis [Berglund T., FEBS Lett. (1994) 351, 145–149] that nicotinamide and/or its metabolites may function as signal transmittors in the response to oxidative stress in plants and that poly(ADP‐ribose)polymerase has a function in the induction of defensive metabolism.