Molecular characterization by RNA‐RNA hybridization of a serotype 8 human rotavirus with “super‐short” RNA electropherotype

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Abstract
A human rotavirus isolate (the 69M strain) with a “super‐short” RNA electropherotype has been classified as serotype 8, a new human serotype [Matsuno et al., 1985] RNA‐RNA hybridzation using 32P‐labeled transcription probes was used to assess the genetic relatedness of the 69M strain to a panel of human and animal rotaviruses. The 69M strain showed a medium level of homology with subgroup I, serotype 2 human rotaviruses with short RNA electropherotypes. However, the 69M strain was not significantly related to any other human rotavirus strains tested in this study, including recently identified serotype 9 strains (represented by the WI61 strain) and subgroup I human rotaviruses with long RNA electropherotypes (represented by the AU‐1 strain) that are shown to be genetically distinct from other human rotavirus strains. Unexpectedly, a medium level of homology was also found between the 69M probe and the double‐stranded RNAs from bovine rotavirus strains (represented by the NCDV strain).