PHOSPHORYLATED CARBOHYDRATE INTERMEDIATES OF THE HUMAN ERYTHROCYTE DURING STORAGE IN ACID CITRATE DEXTROSE. III. EFFECT OF INCUBATION AT 37° C WITH INOSINE, INOSINE PLUS ADENINE, AND ADENOSINE AFTER STORAGE FOR 6, 10, 14, AND 18 WEEKS*

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Abstract
After storage at 4[degree]C for 6, 10, 14 and 18 weeks in acid citrate dextrose, blood was incubated for 1 hour at 37[degree]C with normal saline, inosine, inosine plus adenine, or adenosine, and the phosphorylated carbohydrate intermediates of the erythrocytes were separated on columns of ion-exchange resin. After 6 weeks of storage, very little organic phosphate remained in the control blood. When blood stored for 6 weeks was incubated with inosine, there was resynthesis of a large amount of organic phosphate including adenosine triphosphate; however, after longer periods of storage, incubation with inosine alone was not effective in producing a synthesis of adenylate. When blood which had been stored 18 weeks was incubated with inosine plus adenine or with adenosine, there was still a synthesis of a large amount of organic phosphate, including adenosine triphosphate.