A ROTAVIRUS STAPHYLOCOCCAL CO‐AGGLUTINATION TEST

Abstract
A simple and rapid staphylococcal co-agglutination (SA) test for detection of rotavirus in human fecal specimens is described. A suspension of protein A-containing staphylococci coated with a rabbit hyperimmune serum against the rotavirus strain of calf diarrhea virus was used as reagent. Before the SA test, fecal specimens were absorbed with a suspension of uncoated staphylococci. Of 36 rotavirus immune EM (IEM) positive samples, 34 showed a positive SA test; none of 41 IEM-negative specimens agglutinated the staphylococcal reagent. The SA test was as sensitive as an enzyme-linked immunosorbent assay (Rotazyme) and more sensitive than a latex agglutination test (Rotalex).

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