Glucose, glutamine and inorganic phosphate in early development of the pig embryo in vitro

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Abstract
Pig embryos at the 1- or 2-cell stage (before the ''block'' to development in vitro) were cultured in 8 different media derived from Krebs''-Ringer-bicarbonate medium. A 2 .times. 2 .times. 2 factorial arrangement was used for the treatments, with glucose, glutamine and phosphate being the major effects tested. Embryos were obtained from sows approximately 44-48 h after the observation of oestrus, with the majority being at the 1-cell stage. Embryos from each female were randomly assigned to each treatment. After in-vitro culture, all embryos were scored for the stage of development attained and stained to determine final cell number. Significant effects were evident due to female, glucose, glutamine, a phosphate .times. glucose interaction and a glutamine .times. glucose interaction. None of the media components tested was inhibitory to embryo development. The greatest development (45-60% morula or blastocyst) was achieved with glucose and glutamine (both alone and in combination) in the media, demonstrating that an amino acid can serve as the sole energy source for complete preimplantation embryonic development in vitro.