Two physiological substrate‐specific casein kinases are present in the bovine mammary gland
- 30 January 1989
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 243 (2) , 385-388
- https://doi.org/10.1016/0014-5793(89)80167-x
Abstract
Two species of casein kinase from lactating bovine mammary gland have been identified; a Ca2+‐ and CM‐independent casein kinase and a Ca2+‐ and CM‐dependent casein kinase. The Ca2+‐ and CM‐independent casein kinase phosphorylates previously dephosphorylated αs1‐, β‐ or κ‐casein while the Ca2+‐ and CM‐dependent casein kinase prefers previously dephosphorylated β‐ or κ‐casein as substrates. Two activities are indicated by their substrate specificity, sensitivity to Ca2+ and CM, pH maxima, and differential solubilization by anionic detergents. The presence of a regulated casein kinase in the lactating mammary gland suggests that casein phosphorylation may be a regulator of micelle formation or secretion.Keywords
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