Small, Acid-Soluble Proteins as Biomarkers in Mass Spectrometry Analysis of Bacillu s Spores
Open Access
- 1 February 2003
- journal article
- Published by American Society for Microbiology in Applied and Environmental Microbiology
- Vol. 69 (2) , 1100-1107
- https://doi.org/10.1128/aem.69.2.1100-1107.2003
Abstract
The use of 1 N HCl for extraction of small, acid-soluble proteins (SASP) from different Bacillus spore species was examined. The extracts were analyzed by high-performance liquid chromatography and matrix-assisted laser desorption mass spectrometry and were found to be both qualitatively and quantitatively superior to extraction by acetonitrile-5% trifluoroacetic acid (70:30, vol/vol). Both major and minor α/β- and γ-type SASP were characterized by their molecular masses or tryptic peptide maps and by searches of both protein and unannotated genome databases. For all but 1 pair ( B. cereus T and B. thuringiensis subsp. Kurstaki ) among the 11 variants studied the suites of SASP masses are distinctive, consistent with the use of these proteins as potential biomarkers for spore identification by mass spectrometry.Keywords
This publication has 21 references indexed in Scilit:
- Morphogenesis and Properties of the Bacterial SporePublished by American Society for Microbiology ,2014
- Mechanisms of killing spores of Bacillus subtilis by acid, alkali and ethanolJournal of Applied Microbiology, 2002
- Tandem Mass Spectrometry of Intact Proteins for Characterization of Biomarkers from Bacillus cereus T SporesAnalytical Chemistry, 2001
- Detection of specific Bacillus anthracis spore biomarkers by matrix‐assisted laser desorption/ionization time‐of‐flight mass spectrometryRapid Communications in Mass Spectrometry, 2001
- Analysis of the role of bacterial endospore cortex structure in resistance properties and demonstration of its conservation amongst speciesJournal of Applied Microbiology, 2001
- An α/β-Type, Small, Acid-Soluble Spore Protein Which Has Very High Affinity for DNA Prevents Outgrowth of Bacillus subtilis SporesJournal of Bacteriology, 2001
- Characterization of intact microorganisms by MALDI mass spectrometryMass Spectrometry Reviews, 2001
- Kurstakins: A New Class of Lipopeptides Isolated from Bacillus thuringiensisJournal of Natural Products, 2000
- MECHANISMS FOR THE PREVENTION OF DAMAGE TO DNA IN SPORES OF BACILLUS SPECIESAnnual Review of Microbiology, 1995