Reactivity of EMIT® Serum Barbiturate Assay Reagents

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Abstract
The reactivity of EMIT® serum barbiturate assay reagents toward barbiturate test compounds was investigated. Reactivity was determined by the C5 substituents on the barbiturate ring and O/S substitution (barbituric/ thiobarbituric acid derivatives). Reagents were more reactive toward a 5, 5-substituted barbiturate than toward its hydroxylated analogue. EMIT reactivity could be linearized vs. concentration of test compound, allowing for quantitative analysis in serum or plasma.