FUNCTIONAL-PROPERTIES OF BOVINE IGG1 AND IGG2 - INTERACTION WITH COMPLEMENT, MACROPHAGES, NEUTROPHILS AND SKIN

  • 1 January 1979
    • journal article
    • research article
    • Vol. 38  (2) , 249-256
Abstract
Bovine immunoglobulin (Ig)G subclass (IgG1 and IgG2) antibodies [Ab] fixed bovine complement [C] while only IgG1 fixed guinea pig C in vitro. Similar results were noted when IgG1 and IgG2 Ab were tested by passive cutaneous anaphylaxis (PCA); IgG1 and IgG2 caused PCA in bovine skin while only IgG1 mediated the reaction in rat skin. In precipitation reactions IgG1 Ab to DNP [dinitrophenol] failed to cause precipitation of DNP19-ovalbumin while IgG2 Ab to DNP precipitated DNP19-ovalbumin. IgG1 and IgG2 Ab to ovalbumin precipitated ovalbumin. IgG2 Ab to equine erythrocytes caused phagocytosis by bovine neutrophils and peripheral blood monocytes while IgG1 Ab failed to cause phagocytosis or adherence. Results with peripheral blood monocytes cultured for 7 days demonstrated that IgG1 and IgG2 could mediate phagocytosis.