Prediction of true limiting amino acids using available protein scoring systems†

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Abstract
Mixed diets based on parboiled wheat, milk, rice and lentils were prepared and supplemented with the probable limiting amino acids, either individually or in combination. The amino acid composition, net protein utilization and the true limiting amino acid were determined on each diet. Results were compared with scores obtained using the FAO/WHO (1973) and other possible patterns as reference standards. The 1973 pattern is superior to all others in predicting the true limiting amino acid for test diets in which the first limiting amino acid was either lysine or methionine. The correlation between the scores obtained by use of the FAO/WHO (1973) reference pattern and NPU values with rats was also higher than for any of the other scoring system tested.