Recombinant Fusion Protein and DNA Vaccines Against Foot and Mouth Disease Virus Infection in Guinea Pig and Swine
- 1 January 1999
- journal article
- research article
- Published by Mary Ann Liebert Inc in Viral Immunology
- Vol. 12 (1) , 1-8
- https://doi.org/10.1089/vim.1999.12.1
Abstract
In this study, we provide evidence that a recombinant fusion protein containing β-galactosidase and a tandem repeat peptide of immunogenic dominant epitope of foot-and-mouth disease virus (FMDV) VP1 protein elicits high levels of neutralizing antibody and protects both guinea pigs and swine against infection. Vaccination with this fusion protein induced a FMDV-specific proliferative T-cell response and a neutralizing antibody response. The immunized guinea pigs and swine were protected against FMD type O virus infection. Two DNA plasmids expressing genes of foot-and-mouth disease were constructed. Both plasmids pBO1 and pCO1 contain a signal sequence of the swine immunoglobulin G (IgG) gene and fusion protein gene of pXZ84. The signal sequence and fusion protein gene were under the control of a metallothionein promoter in the case of the pBO1 plasmid and under the control of a cytomegalovirus immediate early promoter in the case of pCO1 plasmid. When pBO1 and pCO1 were inoculated intramuscularly into guinea pigs, both plasmids elicited a neutralizing antibody response and spleen cell proliferation increased following stimulation with FMDV antigen, but animals were not protected from viral challenge.Keywords
This publication has 16 references indexed in Scilit:
- Biological features of genetic immunizationVaccine, 1997
- Vaccination with a Plasmid Vector Carrying the Rabies Virus Glycoprotein Gene Induces Protective Immunity against Rabies VirusVirology, 1994
- New approaches to vaccination against foot-and-mouth diseaseVaccine, 1992
- T Cell-dependent Induction of Antibody against Foot-and-Mouth Disease Virus in a Mouse ModelJournal of General Virology, 1989
- Non-responsiveness to a foot-and-mouth disease virus peptide overcome by addition of foreign helper T-cell determinantsNature, 1987
- Synthesis of fusion proteins with multiple copies of an antigenic determinant of foot-and-mouth disease virus antibodiesGene, 1986
- Protection against foot-and-mouth disease by immunization with a chemically synthesized peptide predicted from the viral nucleotide sequenceNature, 1982
- Cloned Viral Protein Vaccine for Foot-and-Mouth Disease: Responses in Cattle and SwineScience, 1981
- Surface Structure of Foot-and-Mouth Disease VirusJournal of General Virology, 1969