Effects of substrate structure on the kinetics of circle opening reactions of the self-splicing intervening sequence fromTetrahymena thermophila: evidence for substrate and Mg2+binding lnteractions
Open Access
- 1 January 1989
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 17 (1) , 355-371
- https://doi.org/10.1093/nar/17.1.355
Abstract
The self-splicing intervening sequence from the precursor rRNA of Tetrahymena thermophila cyclizes to form a covalently closed circle. This circle can be reopened by reaction with oligonucleotides or water. The kinetics of circle opening as a function of substrate and Mg2+ concentrations have been measured for dCrU, rCdU, dCdT, and H2O addition. Comparisons with previous results for rCrU suggest: (1) the 2' OH of the 5' sugar of a dinucleoside phosphate is involved in substrate binding, and (2) the 2' OH of the 3' sugar of a dimer substrate is involved in Mg2+ binding. Evidently, the binding site for a required Mg ion is dependent on both the ribozyme and the dimer substrate.Keywords
This publication has 32 references indexed in Scilit:
- Kinetics for reaction of a circularized intervening sequence with CU, UCU, CUCU, and CUCUCU: mechanistic implications from the dependence on temperature and on oligomer and magnesium concentrationsBiochemistry, 1988
- Role of Mg2+ in the ribozyme systemFEBS Letters, 1987
- Polymer-supported RNA synthesis and its application to test the nearest-neighbor model for duplex stabilityBiochemistry, 1986
- New reactions of the ribosomal RNA precursor of Tetrahymena and the mechanism of self-splicingJournal of Molecular Biology, 1986
- Metal ion requirements and other aspects of the reaction catalyzed by M1 RNA, the RNA subunit of ribonuclease P from Escherichia coliBiochemistry, 1986
- Reactions of the intervening sequence of the Tetrahymena ribosomal ribonucleic acid precursor: pH dependence of cyclization and site-specific hydrolysisBiochemistry, 1985
- Reverse-phase polystyrene column for purification and analysis of DNA oligomersAnalytical Chemistry, 1984
- Aspects of biochemical catalysisCell, 1984
- The Interaction of Na Ions with Synthetic PolynucleotidesBiopolymers, 1972
- The binding of Mg++ ions to polyadenylate, polyuridylate, and their complexesBiopolymers, 1971