The loss of phenotypic traits by differentiated cells, V. The effect of 5-bromodeoxyuridine on cloned chondrocytes.
- 1 April 1968
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 59 (4) , 1144-1151
- https://doi.org/10.1073/pnas.59.4.1144
Abstract
A pure population of cartilage cells was obtained by individually culturing single [chick] chondrocytes. A single chon-drocyte may yield over 200 colonies. 5-Bromodeoxyuridine added to clonal plates transforms replicating polygonal, matrix-producing cells into exceedingly flattened, fibroblastic cells. Surface areas of transformed cells can be anywhere from 5 to 30 times greater than parental cells. The amount of chondroitin sulfate synthesized by BudR-transformed cells is less than 10 [degree]/o of isogenic control cells. The transformed cells continue to replicate. BudR-supressed chondrocytes have properties in common with dedifferentiated chondrocytes obtained by protracted culturing.This publication has 22 references indexed in Scilit:
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