THE NUTRITION OF BRUCELLAE: UTILIZATION OF SINGLE AMINO ACIDS FOR GROWTH

Publisher Website
Google Scholar
Abstract
Of a number of amino acids and related N compounds surveyed, L- or DL-glutamic acid, L- or DL- (but not D-) asparagine, and L-histidine supported good growth of Brucella abortus, strain 19, when supplied singly as the N source in a chemically defined medium. Apparently, L- and DL-alanine and L-arginine were utilized to a lesser degree. Two CO2-requiring strains of B. abortus were cultivated in the chemically defined medium (with a 10 percent CO2 atmosphere) when L-glutamic acid was supplied as the nitrogen source. B. abortus, strain 19, could be cultivated in a simple medium containing only mineral salts, accessory factors, and L-glutamic acid as the N, C and energy source. L-alanine could partially replace glutamic acid in this respect.