Detection of 500‐nucleotide DNA by laser desorption mass spectrometry
- 1 September 1994
- journal article
- research article
- Published by Wiley in Rapid Communications in Mass Spectrometry
- Vol. 8 (9) , 727-730
- https://doi.org/10.1002/rcm.1290080913
Abstract
We report the first detection of DNA segments as large as 500 nucleotides by matrix‐assisted laser desorption/ionization (MALDI) mass spectrometry, using picolinic acid and 3‐hydroxypicolinic acid mixtures as desorption matrices. The successful detection of 500‐nucleotide DNA indicates that laser mass spectrometry is now emerging as a new biotechnology tool for DNA‐related research. It should be possible to utilize fast detection of large DNA segments by laser mass spectrometry for rapid human genome sequencing.Keywords
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