Failure to Separate Human x- and y-Chromosome Bearing Spermatozoa by Sephadex Gel-Filtration*
- 24 April 2009
- journal article
- Published by Hindawi Limited in Andrologia
- Vol. 10 (3) , 215-217
- https://doi.org/10.1111/j.1439-0272.1978.tb03020.x
Abstract
Human spermatozoa has been filtered on Sephadex gel columns (G 50 fine powder). 50--70% of the spermatozoa put on the gel bed had been recovered within the filtrate, the remaining spermatozoa--not absorbed to gel particles--were found in the upper third of the column. The percentage of motile spermatozoa was higher (80--95%) in the first fraction as compared to the control samples (40--65%). This technique seems to be unsuitable for a successful separation of human x- and y-spermatozoa, an enrichment of x-spermatozoa couldn't be obtained within the first fractions of the filtrate.Keywords
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