Specificity domains distinguish the Ras-related GTPases Ypt1 and Sec4
- 1 April 1993
- journal article
- research article
- Published by Springer Nature in Nature
- Vol. 362 (6420) , 563-565
- https://doi.org/10.1038/362563a0
Abstract
THE essential Ras-related GTPases1,2 Ypt1 and Sec4 act at distinct stages of the secretion pathway in the yeast Saccharomyces cerevisiae: Ypt1 is required for vesicular transport from the endoplasmic reticulum to the Golgi apparatus, whereas Sec4 is required for fusion of secretory vesicles to the plasma membrane3-6. Here we use chimaeras of the two proteins to identify a 9-residue segment of Ypt1 that, when substituted for the analogous segment of Sec4, allows the chimaera to perform the minimal functions of both proteins in vivo. This segment corresponds to loop L7 of the p21ras crystal structure7. Substitution of a 24-residue Ypt1 segment, including the residues just mentioned, together with 12 residues of Ypt1 corresponding to the 'effector region' of p21ras (loop L2; refs 7, 8), transforms Sec4 into a fully functional Ypt1 protein without residual Sec4 function.Keywords
This publication has 25 references indexed in Scilit:
- Engineering hybrid genes without the use of restriction enzymes: gene splicing by overlap extensionPublished by Elsevier ,2003
- Interactions of three domains distinguishing the Ras-related GTP-binding proteins Ypt1 and Sec4Nature, 1993
- Identification of effector-activating residues of GsαCell, 1992
- Hypervariable C-termmal domain of rab proteins acts as a targeting signalNature, 1991
- The GTPase superfamily: conserved structure and molecular mechanismNature, 1991
- A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeastCell, 1988
- Study of a temperature-sensitive mutant of the ras-related YPT1 gene product in yeast suggests a role in the regulation of intracellular calciumCell, 1988
- The cytoplasmic protein GAP is implicated as the target for regulation by the ras gene productNature, 1988
- The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machineryCell, 1988
- A yeast gene encoding a protein homologous to the human c-has/bas proto-oncogene productNature, 1983