Production of Anti-Müllerian Hormone: Another Homology between Sertoli and Granulosa Cells

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Abstract
Anti-Mullerian hormone (AMH) was detected by radioimmunoassay in the follicular fluid of mature bovine ovaries and in incubation medium of bovine granulosa cells. Purification of AMH from 2 independent batches of follicular fluid was achieved with a yield of 11 and 15%, respectively. Both ovarian and control testicular AMH produced near-complete regression of fetal rat Mullerian ducts exposed to it in culture at a final concentration of 200-300 mU/ml and were recognized by the same monoclonal and polyclonal antibodies. Adult mammalian granulosa cells apparently are capable of producing immunoreactive and bioactive AMH at a rate apparently similar to that already demonstrated for mature Sertoli cells. The homologies reported between male and female somatic gonadal cells are discussed.