Detection of anti-Mullerian activity in boar rete testis fluid

Abstract
Boar rete testis fluid [RTF] was tested for its capacity to induce Muellerian regression in 14.5-day-old rat Muellerian ducts. Weak activity was present in crude RTF, but after gel filtration and 5-fold concentration, greater activity was detected in 1 out of 7 pools of the eluted fractions. The biologically active fraction (MW 160,000-310,000) coincided with the elution of authentic labeled anti-Muellerian hormone, obtained from bovine fetal testes. A small amount of anti-Muellerian hormone is evidently still synthesized in post-natal life.