Comparison of Commercial and In-House Real-Time PCR Assays Used for Detection of Mycoplasma pneumoniae
- 1 February 2009
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 47 (2) , 441-444
- https://doi.org/10.1128/jcm.01989-08
Abstract
We tested two commercial and three in-house PCR assays under standardized conditions to detect Mycoplasma pneumoniae . All five procedures were able to demonstrate M. pneumoniae DNA in a concentration comparable to 1 CFU/μl, but the mean crossing points resulted in differences in the concentration of the genome copies of a factor of 20.Keywords
This publication has 24 references indexed in Scilit:
- Epidemiology, clinical manifestations, pathogenesis and laboratory detection ofMycoplasma pneumoniaeinfections: Figure 1FEMS Microbiology Reviews, 2008
- Evaluation of Three Real-Time PCR Assays for Detection of Mycoplasma pneumoniae in an Outbreak InvestigationJournal of Clinical Microbiology, 2008
- Genotyping analysis of Mycoplasma pneumoniae clinical strains in Japan between 1995 and 2005: type shift phenomenon of M. pneumoniae clinical strainsJournal of Medical Microbiology, 2008
- Simultaneous Detection ofChlamydophila pneumoniaeandMycoplasma pneumoniaeby Use of Molecular Beacons in a Duplex Real-Time PCRJournal of Clinical Microbiology, 2008
- Polymerase chain reaction is superior to serology for the diagnosis of acute Mycoplasma pneumoniae infection and reveals a high rate of persistent infectionBMC Microbiology, 2008
- First Report of Macrolide-Resistant Strains and Description of a Novel Nucleotide Sequence Variation in the P1 Adhesin Gene in Mycoplasma pneumoniae Clinical Strains Isolated in France over 12 YearsJournal of Clinical Microbiology, 2007
- Sensitive Detection of Mycoplasma pneumoniae in Human Respiratory Tract Samples by Optimized Real-Time PCR ApproachJournal of Clinical Microbiology, 2007
- Culture-Independent Molecular Subtyping of Mycoplasma pneumoniae in Clinical SamplesJournal of Clinical Microbiology, 2006
- ADP-ribosylating and vacuolating cytotoxin of Mycoplasma pneumoniae represents unique virulence determinant among bacterial pathogensProceedings of the National Academy of Sciences, 2006
- Chlamydia pneumoniae and Mycoplasma pneumoniae in young children from China with community-acquired pneumoniaDiagnostic Microbiology and Infectious Disease, 2005