AVAILABILITY OF METHIONINE IN VARIOUS PROTEIN SUPPLEMENTS AS DETERMINED BY CHICK BIOASSAY

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Abstract
A series of experiments were conducted to obtain estimates of the biological availability of methionine in various protein supplements. The degree of response of the test diets was shown to be linearly related to the level of the supplementary material added, or to some constant function of such levels. Four procedures of bioassay were studied on the basis of slope-ratio technique: (A) weight gain vs. levels of dietary methionine; (B) efficiency of feed utilization vs. levels of dietary methionine; (C) weight gain vs. total available methionine consumed; and (D) gain in weight attributable to intake of test protein methionine vs. methionine intake from the test protein. Method D was the more precise and reliable method as judged by the usual statistical criteria. Using this method the available methionine content of meat, fish, blood, rapeseed, soybean, and feather meals was estimated to be 91.1, 89.9, 66.3, 87.2, 94.7, and 35.0%, respectively.

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