FREQUENCIES OF CENTROMERIC HETEROMORPHISMS OF HUMAN CHROMOSOMES 3 AND 4 AS DETECTED BY QFQ TECHNIQUE: CAN THEY BE IDENTIFIED BY RFA TECHNIQUE?

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Abstract
Normal Caucasians [100] were studied by sequential QFQ [quinacrine bands by fluorescence using quinacrine] and RFA [reverse bands by fluorescence using acridine orange] banding techniques to estimate the type and frequency of heteromorphisms in the centromeric regions of chromosome 3 and 4. Intensity variants were classified into 1 of 5 levels of QFQ banding. QFQ intensity heteromorphisms (.gtoreq. level 3) for chromosomes 3 and 4 were 62 and 15%, respectively. The interrelationship between QFQ and RFA variants were also examined. When the centromere was brilliant by QFQ, it was deep red by RFA; when it was pale by QFQ, it was light red by RFA. A blind coded study could not pick up these color variants by RFA. QFQ banding showed variations of the centromeric regions of chromosomes 3 and 4 while RFA banding failed to demonstrate it. QFQ is evidently the most useful technique in detecting the different intensity levels in the centromeric regions of chromosomes 3 and 4.