Fluorescamine Post-Column Derivatization for the HPLC Determination of Cephalosporins in Plasma and Urine
- 1 October 1988
- journal article
- research article
- Published by Taylor & Francis in Journal of Liquid Chromatography
- Vol. 11 (14) , 2993-3010
- https://doi.org/10.1080/01483918808076775
Abstract
A post-column fluorescamine derivatization procedure is proposed for the determination of cephalosporins having an α-primary amino group in their side chain (cefaclor, cephalexin, cephradine, cefroxadine, cefaloglycine and cefadroxil). The linearity, repeatability and detection limits of fluorescence emission and UV absorption detection are compared under the same chromatographic conditions. Fluorescence detection is about two times more sensitive than UV absorption detection. Application to the determination of these cephalosporins in plasma and urine shows an improved selectivity by comparison with UV detection.This publication has 14 references indexed in Scilit:
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