Site-directed mutagenesis of β-galactosidase (E. coli) reveals that tyr-503 is essential for activity

This publication has 18 references indexed in Scilit:

• [19] Rapid and efficient site-specific mutagenesis without phenotypic selection
  Published by Elsevier ,2004
• m-Fluorotyrosine substitution in β-galactosidase; Evidence for the existence of a catalytically active tyrosine
  Biochemical and Biophysical Research Communications, 1985
• Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors
  Gene, 1985
• Oligonucleotide-Directed Mutagenesis: A Simple Method Using Two Oligonucleotide Primers and a Single-Stranded DNA Template
  DNA, 1984
• Different base/base mismatches are corrected with different efficiencies by the methyl-directed DNA mismatch-repair system of E. coli
  Cell, 1984
• Analysis of a eukaryotic β-galactostdase gene: the N-terminal end of the yeastKluyveromyces lactisprotein shows homology to theEscherichia coli lacZgene product
  Nucleic Acids Research, 1984
• Overproduction of phage Lambda repressor under control of the lac promotor of Escherichia coli
  Molecular Genetics and Genomics, 1976
• Reversible alkylation of a methionyl residue near the active site of β-galactosidase
  Biochemistry, 1972
• pH Dependence of the Activity of β‐Galactosidase from Escherichia coli
  European Journal of Biochemistry, 1971
• The preparation and purification of β-galactosidase from Escherichia coli, ML 308
  Archives of Biochemistry and Biophysics, 1959