Association of human cyclin E with a periodic G1-S phase protein kinase
- 25 September 1992
- journal article
- other
- Published by American Association for the Advancement of Science (AAAS) in Science
- Vol. 257 (5078) , 1958-1961
- https://doi.org/10.1126/science.1329201
Abstract
G1 cyclins control the G1 to S phase transition in the budding yeast, Saccharomyces cerevisiae. Cyclin E was discovered in the course of a screen for human complementary DNAs that rescue a deficiency of G1 cyclin function in budding yeast. The amounts of both the cyclin E protein and an associated protein kinase activity fluctuated periodically through the human cell cycle; both were maximal in late G1 and early S phases. Cyclin E-associated kinase activity was correlated with the appearance of complexes containing cyclin E and the cyclin-dependent kinase Cdk2. Thus, the cyclin E-Cdk2 complex may constitute a human G1-S phase-specific regulatory protein kinase.Keywords
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