Competitive Protein Binding Assay of Camp1Using Thyroid Cytosol
- 1 August 1974
- journal article
- research article
- Published by Taylor & Francis in Analytical Letters
- Vol. 7 (8-9) , 609-619
- https://doi.org/10.1080/00032717408058794
Abstract
We describe here a competitive protein-binding assay (CPBA) of cAMP which employs a crude thyroid cytosol preparation as the ligand-binding reagent. The affinity constant (Ka) of the binding of cAMP to the thyroid receptor varied between 1 and 4 × 109 M−1 in the assay. ATP, ADP and AMP did not interfere in the assay. Cross -reaction of cGMP, 1.8%, and that of cIMP, 36%, were comparable to those observed with previously described CPBAs of cAMP. The range of the assay standard curve was 0.5—20.0 pmoles per tube. Coefficient of variation was 8.3% within an assay and 8.7% between assays. The assay was applied to measurement of cAMP in biologic fluids and tissues. The results were comparable to those obtained with previous methods.Keywords
This publication has 12 references indexed in Scilit:
- A Comparison of Cyclic Nucleotide Levels in Plasma and Cells of Rat and Human Blood1Endocrinology, 1974
- Use of a Modified Radioligand Assay to Measure the Effect of Estradiol on Uterine Adenosine 3',5'–Cyclic MonophosphateEndocrinology, 1973
- Competitive Binding Assay for Adenosine 3′,5′-Monophosphate Employing a Bovine Adrenal Protein: Application to Urine, Plasma and TissuesJournal of Clinical Endocrinology & Metabolism, 1972
- Radioimmunassay for measurement of triiodothyronine in human serumJournal of Clinical Investigation, 1971
- A Simple, Sensitive Protein-binding Assay for Guanosine 3′:5′-MonophosphateProceedings of the National Academy of Sciences, 1971
- A simple and sensitive saturation assay method for the measurement of adenosine 3′:5′-cyclic monophosphateBiochemical Journal, 1971
- An assay for adenosine 3',5'-cyclic monophosphate based on the association of the nucleotide with a partially purified binding proteinBiochemistry, 1970
- A Protein Binding Assay for Adenosine 3′:5′-Cyclic MonophosphateProceedings of the National Academy of Sciences, 1970
- RADIOIMMUNOASSAY FOR THE MEASUREMENT OF ADENOSINE 3′,5′-CYCLIC PHOSPHATEProceedings of the National Academy of Sciences, 1969
- THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONSAnnals of the New York Academy of Sciences, 1949