ISOLATION OF A SUBGROUP 2 ADENOVIRUS FROM CALF WITH WEAK CALF SYNDROME

  • 1 January 1976
    • journal article
    • research article
    • Vol. 40  (1) , 98-103
Abstract
A viral agent, designated Id-1, was isolated from the buffy coat of a calf suffering from weak calf syndrome. The virus replicated on bovine salivary gland cells and caused cytopathic effect within 4 days after infection. Cytopathic effect was characterized by rounding and clumping of cells. Stained preparations of infected monolayers revealed multiple intranuclear inclusions. The agent was resistant to chloroform, ether, trypsin, sodium desoxycholate, oxytetracycline and a pH range of 3-9. The virus was sensitive to 5-iodo-2''-deoxyuridine and to a temperature of 70.degree. C. Cross neutralization tests with [rabbit] Id-1 antiserum and bovine adenovirus type 7 (strain Fukuroi) antiserum resulted in complete neutralization of both viruses with .ltoreq. 4 antibody units of homologous or heterologous antiserum.