Genetic Analysis of H2, the Structural Gene for Phase-2 Flagellin in Salmonella

Abstract
SUMMARY: Non-flagellate H2 mutants were isolated from a phase-2 stable strain, SJW806 H1-gt H2-enxon vh2, a derivative of Salmonella typhimurium. By transductional crosses a deletion map and a recombination map of the H2 gene were made. There are three regions especially rich in nonflagellate mutational sites. By the use of the deletion map, mutational sites of 21 flagellar shape mutants were also determined. Most of them were located at two regions which coincide with two of the three regions rich in non-flagellate mutational sites.