Protein bodies in ray cells of Populus x canadensis Moench ‘robusta’
- 1 January 1988
- journal article
- research article
- Published by Springer Nature in Planta
- Vol. 173 (1) , 31-34
- https://doi.org/10.1007/bf00394483
Abstract
Light- and electron-microscopical investigations revealed distinct intravacuolar protein aggregates of 0.3–0.8 μm in diameter in ray cells of poplar during the dormant season. In semi-thin sections, these bodies showed positive protein staining and enzymatic digestibility with pepsin, indicating their proteinaceous nature. Morphometric measurements showed such protein bodies in 7–13% of the area of the ray-cell lumen. This amount corresponded with the protein content of the wood determined biochemically, e.g. 2.0–5.0 μg·mg-1 dry weight. Polyacrylamide gel electrophoresis of the total protein fraction extracted from wood showed prominent polypeptide species with an apparent molecular weight of 30–32 kilodaltons. The results indicate considerable protein storage in ray cells, especially in the form of protein-storage vacuoles.Keywords
This publication has 15 references indexed in Scilit:
- Changes in carbohydrates and ultrastructure in xylem ray cells ofPopulus in response to chillingProtoplasma, 1987
- Sambucus nigra agglutinin is located in protein bodies in the phloem parenchyma of the barkPlanta, 1986
- Plasmodesmatal frequency and radial translocation rates in ray cells of poplar (Populus x canadensis Moench ?robusta?)Planta, 1986
- Hydrolysis of Intracellular Proteins in Vacuoles Isolated from Acer pseudoplatanus L. CellsPlant Physiology, 1985
- Comparative Distribution and Metabolism of Xylem-Borne Amino Compounds and Sucrose in Shoots of Populus deltoidesPlant Physiology, 1985
- Glutamine Transfer from Xylem to Phloem and Translocation to Developing Leaves of Populus deltoidesPlant Physiology, 1985
- Origin and development of protein bodies in cotyledons of Vicia fabaPlanta, 1983
- Development of protein bodies in oat starchy endospermCanadian Journal of Botany, 1983
- Nutrient reserves in roots of fruit trees, in particular carbohydrates and nitrogenPlant and Soil, 1983
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970