Rapidly denoising pyrosequencing amplicon reads by exploiting rank-abundance distributions
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Open Access
- 1 September 2010
- journal article
- research article
- Published by Springer Nature in Nature Methods
- Vol. 7 (9) , 668-669
- https://doi.org/10.1038/nmeth0910-668b
Abstract
We developed a fast method for denoising pyrosequencing for community 16S rRNA analysis. We observe a 2–4 fold reduction in the number of observed OTUs (operational taxonomic units) comparing denoised with non-denoised data. ~50,000 sequences can be denoised on a laptop within an hour, two orders of magnitude faster than published techniques. We demonstrate the effects of denoising on alpha and beta diversity of large 16S rRNA datasets.Keywords
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